Cu2+对序批式反应器中活性污泥胞外聚合物产量及其组分的影响     

李姗姗  朱四富  马丙瑞  于娜玲  赵长坤  信帅帅  潘云浩  吴淑妍  武原原  高孟春 《中国海洋大学学报(自然科学版)》2020,(2):107-115

研究了长期暴露条件下Cu2+对序批式反应器(SBR)性能及其活性污泥胞外聚合物(EPS)特性的影响。结果表明,进水中加入10 mg·L-1的Cu2+后,在SBR运行的第16~55天,COD和NH+4-N的去除率保持稳定;在第56~75天,COD和NH+4-N的平均去除率与进水Cu2+浓度为0 mg·L-1时相比分别下降了3.88%和6.41%。浓度为10 mg·L-1的Cu2+长期作用下,活性污泥中EPS、松散附着EPS(LB-EPS)和紧密附着EPS(TB-EPS)产量及LB-EPS和TB-EPS中蛋白质(PN)含量增加。傅里叶变换红外光谱分析表明10 mg·L-1 Cu2+的长期暴露导致TB-EPS中PN的C=O键、N-H键和C-O键相对含量降低。X射线光电子能谱(XPS)测试结果显示在10 mg·L-1 Cu2+长期暴露条件下,LB-EPS和TB-EPS中元素Cu和O百分含量增加。  相似文献   

基于参与式制图方法的景观服务评估与空间结构研究     

朱亚茹  高峻  邴振华  张中浩  付晶 《地球信息科学学报》2020,22(5):1106-1119

景观服务评估核心是人与环境之间复杂的动态关系,基于利益相关者的参与式制图方法是建立景观服务分类体系和量化指标的有效途径。本文采用参与式制图方法,以九寨沟自然保护区为例,根据景观服务分类研究成果,并结合九寨沟自然资源特点和景观服务使用者半结构式访谈数据,提出了适用于九寨沟的景观服务指标体系。本研究方法以管理局工作人员、居民和游客等服务使用者作为参与式调研对象,分别就其需求和景观服务感知程度进行半结构式访谈,并利用GIS技术将数据空间位置和功能属性结合,得到九寨沟有3大类景观服务和17个相关指标,以及737个景观服务点。通过研究结果可知,当地村落建筑与众多景观服务指标（包括观鸟场所、野营旅游、民俗与节庆等13个指标）具有极强的空间关联性。同时,景观服务使用者感知的景观价值在空间上属于聚集分布形式,主要围绕着开放生态旅游的3条沟谷分布,并向外围扩散。其中景观服务的“高—高”空间聚集区主要分布在沟口等与人类需求密切联系的区域,而景观服务的“低—低”空间聚集区则主要分布在原始森林等与人类居住、活动相距较远的区域。综上,基于参与式制图方法建立的景观服务分类和空间分析,发现九寨沟景观服务的空间位置与使用者紧密相关,其景观价值的空间结构特征是服务使用者与环境长期互动的结果,该结果可以用来引导并进一步优化九寨沟的功能分区规划。  相似文献   

Supplementation of Yeast Extract to Practical Diet Improves the Growth,Anti-Oxidative Capacity and Intestinal Morphology of Shrimp Litopenaeus vannamei     

MA Shuoli  WANG Xiaoxia  GAO Weihua  ZHANG Wenbing  MAI Kangsen 《中国海洋大学学报(英文版)》2019,(4)

Two control diets based on the commercial formula were designed to contain high(27%, D1) and low(22%, D2) levels of fish meal, respectively. Into D2, 500, 1000 and 1500 mg kg~(-1) of yeast extract were added, respectively, yielding three experimental diets(YE1 through YE3). Shrimp(initial body weight 0.30 g ± 0.02 g) were fed with the experimental diets, five tanks each diet and 30 shrimp individuals each tank, for 8 weeks, and then challenged with Vibrio parahaemolyticus. The results showed that the specific growth rate(SGR) of shrimp in D2 was significantly lower than that of shrimp in D1(P 0.05). The SGR of shrimp in YE3 was similar to that of shrimp in D1. The feed intake of shrimp was similar between D1 and D2. The feed conversion ratio and protein efficiency ratio of shrimp were similar among all diets(P 0.05). YE significantly improved the activity of glutathione S-transferase. The concentration of glutathione(GSH) and the total serum anti-oxidative capacity(T-AOC) of D1 were significantly higher than those of shrimp feeding other diets(P 0.05). The content of serum malondialdehyde of shrimp feeding YE2 and YE3 was significantly lower than that of shrimp feeding D2(P 0.05). The thickness of intestine muscular layer of shrimp feeding YE1 and YE2 was similar to that of shrimp feeding D1. The shrimp feeding YE1 showed the highest villus height of intestine among all groups. The cumulative mortality after challenging was similar among all groups(70.00%–86.67%)(P 0.05). In conclusion, 1000–1500 mg kg~(-1) of YE was suggested to be supplemented into the practical diets to improve the growth, anti-oxidative capacity and intestinal morphology of shrimp L. vannamei.  相似文献   

Case Study of Fog Predictability for an Event with Cold-Front Synoptic Pattern     

HU Huiqin  HUANG Fei  ZHANG Shaoqing  RUAN Chengqing  GAO Shanhong  LI Pengyuan 《中国海洋大学学报(英文版)》2019,(2)

Fog has recently become a frequent high-impact weather phenomenon along the coastal regions of North China. Accurate fog forecasting remains challenging due to limited understanding of the predictability and mechanism of fog formation associated with synoptic-scale circulation. One frequent synoptic pattern of fog formation in this area is associated with cold front passage(cold-front synoptic pattern, CFSP). This paper explored the predictability of a typical CFSP fog event from the perspective of analyzing key characteristics of synoptic-scale circulation determining fog forecasting performance and the possible mechanism. The event was ensemble forecasted with the Weather Research and Forecasting model. Two groups of ensemble members with good and bad forecasting performance were selected and composited. Results showed that the predictability of this case was largely determined by the simulated strengths of the cold-front circulation(i.e., trough and ridge and the associated surface high). The bad-performing members tended to have a weaker ridge behind a stronger trough, and associated higher pressure over land and a weaker surface high over the sea, leading to an adverse impact on strength and direction of steering flows that inhibit warm moist advection and enhance cold dry advection transported to the focus region. Associated with this cold dry advection, adverse synoptic conditions of stratification and moisture for fog formation were produced, consequently causing failure of fog forecasting in the focus region. This study highlights the importance of accurate synoptic-scale information for improved CFSP fog forecasting, and enhances understanding of fog predictability from perspective of synoptic-scale circulation.  相似文献   

基于遥感水边线的潮滩面积提取方法研究          下载免费PDF全文

张媛媛  高志强  刘向阳  许宁 《海洋开发与管理》2018,35(3):56-61

潮滩作为动态变化的后备土地资源,对其研究具有重要意义。文章以江苏省如东县为研究区,使用环境资源卫星(HJ1A/1B)影像为数据源,应用面向对象技术创建分类规则,实现批量半自动提取水边线,随后利用DSAS软件处理水边线集获得潮位点集,基于最外边界法实现高(低)潮位点的提取,进而获得潮滩范围,估算的如东县潮滩面积为55 182hm~2。最后利用误差矩阵进行精度验证(Kappa系数为0.945)。结果表明:该方法对于潮滩面积提取具有适用性,无论在空间域还是时间域都具有一定的推广性。  相似文献   

波浪作用下单桩基础周围海床液化机制研究   总被引：1，自引：1，他引：0  

隋倜倜  张弛  高玉峰  郑金海 《海洋工程》2018,36(4):88-96

建立波浪作用下单桩周围三维海床动力响应模型,考虑自重影响下的海床长时间固结过程。采用已有物理模型试验数据对模型进行验证,证实其具有较好的适用性。模拟波浪作用下单桩周围三维海床液化区域,通过定量分析超孔隙水压力和土体初始有效应力的变化,讨论单桩插入深度对海床液化的影响机制。研究表明,单桩插入深度发生变化时,土体初始有效应力对海床液化的影响要大于超孔隙水压力,且影响程度随着插入深度的增加而逐渐增大。  相似文献   

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy     

CHAN Zhuhua  CHEN Xinglin  HOU Yanping  GAO Boliang  ZHAO Chungui  YANG Suping  ZENG Runying 《海洋学报(英文版)》2018,(2)

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.  相似文献   

多遮挡环境下BDS/GPS组合动态差分处理算法及性能分析     

王云鹏  石波  王胜利  刘猛奎  张倩  高 《大地测量与地球动力学》2018,38(3):276-281

研究BDS/GPS组合载波相位动态差分定位算法，讨论加入BDS后对模糊度固定的影响，针对部分区域有较严重信号遮挡的实测动态数据，分别进行单GPS和BDS/GPS组合解算。结果表明，相比单GPS，加入BDS后增加了共视卫星数目，改善了卫星几何结构，模糊度解算成功率和可靠性得到明显提高。多遮挡环境下测试数据显示，BDS/GPS组合的定位精度远高于单GPS，东向和北向精度优于8 mm、天向为16 mm，而单GPS东向仅为3 cm、北向和天向约8 cm。  相似文献   

多氯联苯(Aroclor 1242)胁迫下鲤鱼肝脏组织氧化应激   总被引：1，自引：0，他引：1  

王仁君  王建国  丁宁  孙萌  高配科 《海洋与湖沼》2018,49(6):1286-1293

本文在实验室条件下通过静态水质染毒方式在生理生化和转录水平上探究了我国重要经济鱼种鲤鱼(Cyprinus carpioio)对多氯联苯Aroclor 1242的氧化应激:鲤鱼肝脏组织抗氧化酶[超氧化物歧化酶(SuperoxideDismutase,SOD)和过氧化氢酶(Catalase,CAT)]活性、脂质过氧化产物丙二醛(Malonydialdehyde,MDA)含量、相关抗氧化酶编码基因(Cu/Zn-SOD、Mn-SOD、CAT)、应激蛋白HSP70、转录因子Nrf2和芳烃受体AhR2基因表达。结果表明:Aroclor 1242胁迫下,鲤鱼肝脏组织抗氧化酶活性表现出低浓度促进高浓度抑制的趋势;肝脏组织丙二醛含量显著升高,其中390μg/L暴露组鲤鱼肝脏组织MDA含量在第6d高于对照组92.1%(P0.01),表明肝脏组织发生脂质过氧化,膜系统受到损伤;鲤鱼肝脏组织中Cu/Zn-SOD、Mn-SOD、CAT、HSP70、Nrf2和AhR2编码基因表达出现不同程度下调。本文从酶学和基因转录水平上揭示了Aroclor1242胁迫下鲤鱼肝脏组织细胞的氧化应激。  相似文献   

鲁西大汶口地区全新世临沂组沉积物粒度分布特征及地质意义          下载免费PDF全文

高明波 《山东国土资源》2018,34(7)

出露于大汶口盆地的临沂组为一套第四系松散堆积物,上部以土黄色、灰黄色含砂质粉砂、粉砂质粘土为主,下部为砂砾石层,是大汶口盆地内河流演化过程中的沉积响应和物质记录。通过对临沂组进行研究,可以反演大汶口盆地第四纪以来的沉积区水动力环境、物质运移方式和沉积环境。该文在区域地质调查工作的基础上,结合粒度分析频率曲线、概率累积曲线、粒度参数和沉积物宏观特征,对临沂组的沉积相、沉积环境和沉积韵律进行了研究。研究表明,大汶口地区临沂组下部为河床亚相边滩沉积微相,上部为河漫亚相河漫滩沉积微相,经历了由简单高能→复杂中能-中低能→简单中低能→复杂中低能的水动力沉积环境变化。另外依据岩石组合特征,将其划分为4个明显的沉积韵律和1个不明显的沉积韵律组。  相似文献